饲料磷真消化率不同的山羊瘤胃微生物差异性研究

doi:10.11843/j.issn.0366-6964.2018.12.014

Abstract

Abstract:

The objective of this study was to compare the structure and composition of rumen microorganism of goats with different true digestibility of feed phosphorus using Illumina MiSeq sequencing technology. Twenty-eight Nubian Black goats, aged 10 months on average and weighed (24.25±2.47) kg, were selected as the experimental animals. After two digestion tests, the content of phosphorus in feed and feces was determined, and the true digestibility of phosphorus in all goat feed was calculated by differential method. Among them, 6 goats with high phosphorus true digestibility were selected as HP group and 6 goats with low phosphorus true digestibility as LP group. Rumen fluid from each goat in HP and LP groups was collected and then total genomic DNA was extracted. High variable region of bacterial 16S rRNA gene was amplified by PCR using bacterial universal primers. The amplicon sequence data was analyzed by the Illumina MiSeq sequencing platform, and bioinformatics analysis was performed using QⅡME software. The results showed that:1) The content of phosphorus in blood of goats in HP group was significantly higher than that in LP group (P<0.05). 2) At the phylum level, Firmicutes and Bacteroidetes were the dominant bacteria in the rumen microorganisms of goats in the two groups; 3)At the genus level, the relative abundances of 10 genus were significantly different between the two groups (P<0.05). Only the relative abundances of Prevotella, Desulfovibrio and Selenomonas in HP group were significantly higher than that in LP group(P<0.05), but the relative abundances of Ruminococcaceae_UCG-010, Ruminococcus_2, Ruminococcaceae_NK4A214_group, Saccharofermentans, Ruminococcaceae_UCG-014, Butyrivibrio and Clostridium were significantly lower in HP group than that in LP group (P<0.05). The relative abundance of rumen microorganisms was significantly correlated with the phosphorus true digestibility in goats. There are significant differences in structure and composition of rumens microorganisms among goats with different phosphorus true digestibility.

CLC Number:

S827.5

JIN Lei, WANG Li-zhi, GU Ke, WANG Zhi-sheng, XUE Bai, PENG Quan-hui. Differences in Rumen Microbes of Goats with Different Phosphorus True Digestibility[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2652-2663.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.12.014

https://www.xmsyxb.com/EN/Y2018/V49/I12/2652

References

[1] 何万领,李晓丽,李旺,等.磷源与磷水平对蛋鸡生产性能及钙、磷、氮代谢的影响[J].畜牧兽医学报,2018,49(5):1061-1073.
HE W L,LI X L,LI W,et al.Effects of phosphorus forms and levels on the production performance and metabolism of calcium,phosphorus and nitrogen in laying hens[J].Acta Veterinaria et Zootechnica Sinica,2018,49(5):1061-1073.(in Chinese)
[2] 王浩,林昌龙,周广琛,等.陕北白绒山羊断奶羔羊钙磷需要量的研究[J].畜牧兽医学报,2018,49(1):121-130.
WANG H,LIN C L,ZHOU G C,et al.Study on calcium and phosphorus requirement of weaned lamb in Shaanbei white cashmere goat[J].Acta Veterinaria et Zootechnica Sinica,2018,49(1):121-130.(in Chinese)
[3] 张浩,聂海涛,马铁伟,等.20~35 kg杜湖F₁代母羔常量元素净维持和生长需要量的研究[J].畜牧兽医学报,2018,49(1):111-120.
ZHANG H,NIE H T,MA T W,et al.The net macromineral requirements for maintenance and growth of Dorper×Hu crossbred F₁ ewe lambs from 20 to 35 kg[J].Acta Veterinaria et Zootechnica Sinica,2018,49(1):111-120.(in Chinese)
[4] LEI X G,STAHL C H.Nutritional benefits of phytase and dietary determinants of its efficacy[J].J Appl Anim Res,2000, 17(1):97-112.
[5] KEBREAB E,HANSEN A V,STRATHE A B.Animal production for efficient phosphate utilization:from optimized feed to high efficiency livestock[J].Curr Opin Biotechnol,2012,23(6):872-877.
[6] LEYTEM A B,THACKER P A.Phosphorus utilization and characterization of excreta from swine fed diets containing a variety of cereal grains balanced for total phosphorus[J].J Anim Sci,2010,88(5):1860-1867.
[7] 李岚捷,成述儒,刁其玉,等.不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响[J].畜牧兽医学报,2017,48(12):2347-2357.
LI L J,CHENG S R,DIAO Q Y,et al.Effects of diets with different nfc/ndf levels on the rumen fermentation parameters and bacterial community in male calves[J].Acta Veterinaria et Zootechnica Sinica,2017,48(12):2347-2357.(in Chinese)
[8] 王尧悦,赵钊艳,王兴涛,等.日粮营养水平对150~180日龄滩羊瘤胃相关微生物菌群数量、pH和VFA含量的影响[J].畜牧兽医学报,2016,47(10):2060-2070.
WANG Y Y,ZHAO Z Y,WANG X T,et al.Effect of dietary nutrient levels on the number of related microbes,pH and VFA levels in rumen of tan sheep aged from 150 to 180 days[J].Acta Veterinaria et Zootechnica Sinica,2016,47(10):2060-2070.(in Chinese)
[9] 沈莎莎,杨红建,张晓明.瘤胃微生物植酸酶的分类与酶学性质研究进展[J].畜牧兽医学报,2011,42(12):1655-1660.
SHEN S S,YANG H J,ZHANG X M.Research advance in classification and enzymological characteristics of microbial phytase in the rumen[J].Acta Veterinaria et Zootechnica Sinica,2011,42(12):1655-1660.(in Chinese)
[10] ALONSO V,CAVAGLIERI L,RAMOS A J,et al.Modelling the effect of pH and water activity in the growth of Aspergillus fumigatus isolated from corn silage[J].J Appl Microbiol,2017,122(4):1048-1056.
[11] FAN M Z,ARCHBOLD T,SAUER W C,et al.Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs[J].J Nutr,2001,131(9):2388-2396.
[12] JULÁK J,STRÁNSKÁ E,PROCHÁZKOVÁ-FRANCISCI E,et al.Blood cultures evaluation by gas chromatography of volatile fatty acids[J].Med Sci Monit,2000,6(3):605-610.
[13] GUO W,LI Y,WANG L Z,et al.Evaluation of composition and individual variability of rumen microbiota in yaks by 16S rRNA high-throughput sequencing technology[J].Anaerobe,2015,34:74-79.
[14] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QⅡME allows analysis of high-throughput community sequencing data[J]. Nat Methods,2010,7(5):335-336.
[15] EDGAR R C,HAAS B J,CLEMENTE J C,et al.UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics, 2011,27(16):2194-2200.
[16] HUSE S M,WELCH D M,MORRISON H G,et al.Ironing out the wrinkles in the rare biosphere through improved OTU clustering[J].Environ Microbiol,2010,12(7):1889-1898.
[17] EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.
[18] QUAST C,PRUESSE E,YILMAZ P,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nucleic Acids Res,2013,41(D1):D590-D596.
[19] FU L L,JIANG B H,LIU J L,et al.Genome sequence analysis of a flocculant-producing bacterium,Paenibacillus shenyangensis[J]. Biotechnol Lett,2016,38(3):447-453.
[20] BRAVO D,SAUVANT D,BOGAERT C,et al.Quantitative aspects of phosphorus excretion in ruminants[J].Reprod Nutr Dev, 2003,43(3):285-300.
[21] 刘静波,杨跃奎,何健.日粮磷水平对线性回归法测定磷真消化率的影响[J].畜牧兽医学报,2014,45(4):572-577.
LIU J B,YANG Y K,HE J.Effect of dietary phosphorus levels on the true phosphorus digestibility estimated by the linear regression method[J].Acta Veterinaria et Zootechnica Sinica,2014,45(4):572-577.(in Chinese)
[22] 刘正群,张祖翔,陈亮,等.生长猪基础饲粮组成对磷酸氢钙和磷酸二氢钙中磷的全肠道真消化率的影响[J].动物营养学报,2016,28(8):2542-2550.
LIU Z Q,ZHANG Z X,CHEN L,et al.Effects of basal diet composition on true total tract digestibility of phosphorus in dicalcium phosphate and monocalcium phosphate for growing pigs[J].Chinese Journal of Animal Nutrition,2016, 28(8):2542-2550.(in Chinese)
[23] ABBASI F,LIU J B,ZHANG H F,et al.Effects of dietary total phosphorus concentration and casein supplementation on the determination of true phosphorus digestibility for broiler chickens[J].Ital J Anim Sci,2018,17(1):135-144.
[24] REID R L,FRANKLIN M C,HALLSWORTH E A.The utilization of phytate phosphorus by sheep[J].Aust Vet J,1947,23(6):136-140.
[25] SELLE P H,COWIESON A J,RAVINDRAN V.Consequences of calcium interactions with phytate and phytase for poultry and pigs[J].Livest Sci,2009,124(1-3):126-141.
[26] MUTUCUMARANA R K,RAVINDRAN V,RAVINDRAN G,et al.Measurement of true ileal phosphorus digestibility in maize and soybean meal for broiler chickens:comparison of two methodologies[J].Anim Feed Sci Technol,2015,206:76-86.
[27] RICHARDSON E C,HERD R M.Biological basis for variation in residual feed intake in beef cattle.2.Synthesis of results following divergent selection[J].Aust J Exp Agric,2004,44(5):431-440.
[28] AZEEM M,RIAZ A,CHAUDHARY A N,et al.Microbial phytase activity and their role in organic p mineralization[J].Arch Acker Pflanzenbau Bodenkd,2015,61(6):751-766.
[29] KINCAID R L,GARIKIPATI D K,NENNICH T D,et al.Effect of grain source and exogenous phytase on phosphorus digestibility in dairy cows[J].J Dairy Sci,2005,88(8):2893-2902.
[30] KNOWLTON K F,TAYLOR M S,HILL S R,et al.Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase[J].J Dairy Sci,2007,90(9):4356-4360.
[31] YANKE L J,BAE H D,SELINGER L B,et al.Phytase activity of anaerobic ruminal bacteria[J].Microbiology, 1998, 144(6):1565-1573.
[32] SOTAK-PEPER K M,GONZáLEZ-VEGA J C,STEIN H H.Effects of production area and microbial phytase on the apparent and standardized total tract digestibility of phosphorus in soybean meal fed to growing pigs[J].J Anim Sci,2016,94(6):2397-2402.
[33] CASAS G A,STEIN H H.Effects of microbial phytase on the apparent and standardized total tract digestibility of phosphorus in rice coproducts fed to growing pigs[J].J Anim Sci,2015,93(7):3441-3448.
[34] GONZÁLEZ-VEGA J C,WALK C L,STEIN H H.Effect of phytate,microbial phytase,fiber,and soybean oil on calculated values for apparent and standardized total tract digestibility of calcium and apparent total tract digestibility of phosphorus in fish meal fed to growing pigs[J].J Anim Sci,2015,93(10):4808-4818.
[35] HAROS M,BIELECKA M,SANZ Y.Phytase activity as a novel metabolic feature in Bifidobacterium[J].FEMS Microbiol Lett,2005,247(2):231-239.
[36] CHENG C W,LIM B L.Beta-propeller phytases in the aquatic environment[J].Arch Microbiol,2006,185(1):1-13.
[37] GULATI H K,CHADHA B S,SAINI H S.Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil[J].J Ind Microbiol Biotechnol,2007,34(1):91-98.
[38] GODOY S,CHICCO C F.In vitro phytase activity of rumen microorganism and in situ degradation of fibrous substrate in sheep fed different feeding regimes[J].Zootec Trop,2005,23(1):61-68.
[39] JAMI E,MIZRAHI I.Composition and similarity of bovine rumen microbiota across individual animals[J].PLoS One,2012, 7(3):e33306.
[40] POPE P B,DENMAN S E,JONES M,et al.Adaptation to herbivory by the Tammar wallaby includes bacterial and glycoside hydrolase profiles different from other herbivores[J].Proc Natl Acad Sci U S A,2010,107(33):14793-14798.
[41] DE OLIVEIRA M N V,JEWELL K A,FREITAS F S,et al.Characterizing the microbiota across the gastrointestinal tract of a Brazilian Nelore steer[J].Vet Microbiol,2013,164(3-4):307-314.
[42] MAO S Y,ZHANG M L,LIU J H,et al.Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle:membership and potential function[J].Sci Rep,2015,5:16116.
[43] CHEN Y H,PENNER G B,LI M J,et al.Changes in bacterial diversity associated with epithelial tissue in the beef cow rumen during the transition to a high-grain diet[J].Appl Environ Microbiol,2011,77(16):5770-5781.

Differences in Rumen Microbes of Goats with Different Phosphorus True Digestibility

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	YAN Qiongxian, CHEN Wenxun, HUI Haoyang, PENG Can, TANG Shaoxun, ZHOU Xiaoling, TAN Zhiliang. Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1109-1123.
[2]	Lü Xiaokang, XIE Biao, HUANG Wenqin, WANG Shuyan, BI Yanliang, TAO Hui, CUI Kai, DIAO Qiyu, ZHANG Naifeng. Effects of Early Feeding on Rumen and Small Intestine Morphology of Goat Kids [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1006-1015.
[3]	WANG Hao, LIN Chang-long, ZHOU Guang-chen, CHEN Yu-lin, YANG Yu-xin. Study on Calcium and Phosphorus Requirement of Weaned Lamb in Shaanbei White Cashmere Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 121-130.